How to apply technical guidelines in OneLab
Define your guidelines:
Decide which technical guidelines you want to use (see the list below) and in which protocol step.
Modify actions in the Protocol designer:
Go to the protocol step you want to modify (pipette-based or device-based) and click on it to edit it if required.
Scroll down to the Guidelines section of the protocol step.
Write the technical guideline in the Guidelines section and click Save step.
Execute your protocol; the guidelines will be applied ✔️
List of technical guidelines
⚠️ Important: Only one type of technical guideline can be used per action.
🧰 Force connected device selection in OneLab:
Available since: OneLab 1.22.1
Purpose: Define a preferred connected device for a specific step (e.g., Heating action). Or to use several Connected Devices of the same type (i.e., two or more Heater-Shaker+, Peltier+, Shaker+, etc.) in the same protocol without the need for a nested action.
Settings: Add the device's serial number to the guidelines section.
Example:
518.5507.1587H
Notes:
If the device is incompatible or not ready, OneLab will default to automatic device selection.
If a serial number is used in one step but not in the subsequent steps, the specified device is used for all subsequent steps.
Multiple devices of the same type can be utilized during an experiment. For example, with two Peltier+ devices, one can heat while the other preheats, allowing seamless transitions and reducing the waiting time of cooldown.
💧 Force pipette selection in OneLab/Andrew+:
Available since: OneLab 1.19.4
Purpose: Define a preferred pipette for a specific step in OneLab. Andrew+ will prioritize the use of the specified pipette.
Settings and list of pipette codes:
1ch-10
1ch-120
1ch-300
1ch-1000
1ch-5000
1ch-10000
8ch-10
8ch-120
8ch-300
8ch-1200
12ch-10
12ch-120
12ch-300
12ch-1200*12-ch pipettes are only usable with Pipette+
Example:
You can either add only one pipette code or a list:
8ch-300,8ch-120,1ch-300
To prioritize multichannel pipettes, add their codes at the top of the list.
Notes:
OneLab will prioritize the order defined as your preference.
If the defined pipette is incompatible with source or destination labware, volumes, etc., OneLab will fall back on the automatic pipette selection.
To restrict the choice of pipette to the one listed in the action, you need to add the code "only" for the corresponding pipette:
only 8ch-120
In this case, if the defined pipette is not on the device tool rack, the execution will be blocked and an error will be displayed on the setup selection screen.
⌛ Pause during pipetting in OneLab:
Available since: OneLab 1.17.0
Purpose: Andrew+ will perform pauses at specific moments during pipetting.
Settings: 4 settings are available, each for a specific moment of the pipetting:
Aspiration | Aspiration | Dispensing | Dispensing |
aspiration, then waiting for x time in the liquid | aspiration, then go on top of the consumable, then waiting for x time | dispensing, then waiting for x time in the liquid | dispensing, then go on top of the consumable, then waiting for x time |
|
|
| |
waiting during aspirating: <time to wait>
| waiting after aspirating: <time to wait> | waiting during dispensing: <time to wait> | waiting after dispensing: <time to wait> |
The <time to wait> can be used with the following units of time:
msec (milliseconds)
sec (seconds)
min (minutes)
Examples:
waiting during aspirating: 30sec
waiting after dispensing: 5sec
Notes:
This adjustment will apply to every pipetting of an action.
These pauses CANNOT be combined.
♻️ Return tips to Tis Insertion System Domino - Andrew+:
Available since: OneLab 1.14.0
Purpose: Andrew+ returns tips to their original position in the Tis Insertion System Domino instead of ejecting them into waste.
Code:
put tips back
Notes:
This code must be inserted in a Notification action (in the protocol steps).
The behavior will apply to all pipetting-based actions of the entire protocol.
This action is automatically disabled when "avoid overfly of clean tips" is selected. Both are incompatible.