NAD+ is a naturally occurring molecule your cells use for energy, repair, and keeping mitochondria running smoothly. Levels tend to decline with age and stress, which is why many wellness and longevity enthusiasts look to restore or support it.

Daily subcutaneous (SQ) NAD+ has become a lower-dose “maintenance style” approach—much gentler than the high-dose IV protocols you may have heard about.

Add 3.0 mL bacteriostatic water → 166.7 mg/mL concentration.

This dilution keeps your injection volumes small and makes measuring simple.

NAD+ is a naturally occurring coenzyme essential for cellular energy production, mitochondrial function, and metabolic regulation. It plays a central role in hundreds of biochemical reactions that support ATP production, DNA repair, and cellular signaling pathways.

Because NAD+ levels naturally decline with age and metabolic stress, research and wellness protocols often explore strategies to support optimal NAD+ availability and cellular metabolism.

NAD+ functions as a critical electron carrier in cellular metabolism, helping convert nutrients into usable energy.

It supports several key biological processes:

• Mitochondrial energy production (ATP)
• DNA repair and cellular maintenance
• Metabolic signaling pathways
• Sirtuin activation, which is studied in longevity and metabolic research

Through these mechanisms, NAD+ is often explored for its role in supporting energy levels, metabolic health, and cognitive performance.

• Add 5.0 mL bacteriostatic water → final concentration 100 mg/mL

• This concentration allows convenient and accurate measurement of NAD+ doses using a standard insulin syringe.

• 30–50 mg per injection

• Many protocols begin at 30 mg to assess individual tolerance.

• Doses between 30–50 mg per injection are commonly referenced in NAD+ protocols, depending on individual goals and response.

At 100 mg/mL concentration

• 1 unit = 1 mg on a U-100 insulin syringe

Lyophilized:

• Store frozen at −20 °C (−4 °F)

Reconstituted:

• Refrigerate at 2–8 °C (35.6–46.4 °F)
• Use within 2–4 weeks

Frequency: 2–3× per week

Administration: Subcutaneous (SubQ) or Intramuscular (IM)

Gradual dose increases may help improve comfort and tolerability.

Approximately 10 injections at 50 mg

• 1 per injection

• 5 mL per vial

• 2 per injection (vial + skin)

Goal:

Support cellular energy production, mitochondrial health, and metabolic signaling pathways.

Structure:

• Subcutaneous or intramuscular injections

• 30–50 mg per dose

• 2–3 injections per week

This structure is commonly used in wellness and metabolic-support protocols.

Before injecting:

• Wash hands
• Clean vial stopper with alcohol
• Clean injection site
• Draw dose and remove air bubbles

Injection method:

Subcutaneous (SubQ)
Pinch skin and insert needle at 45–90°

Intramuscular (IM)
Inject into deltoid, thigh, or glute

Inject slowly, remove needle, and dispose of it in a sharps container.

• Cellular energy metabolism support
• Mitochondrial function support
• Support for metabolic and cognitive health
• Research interest in healthy aging pathways

• Temporary warmth or flushing sensation
• Mild pressure or tightness if injected too quickly
• Injection site irritation

Administering injections slowly often improves comfort.

To get the most from NAD+ protocols:

Disclaimer:

All peptide products sold on this website are for research, laboratory, and educational purposes only. They are not approved by the FDA or Health Canada, are not intended for human consumption, and should not be used for medical, cosmetic, or therapeutic purposes unless specifically prescribed by a licensed healthcare professional.

The information provided on this site is for informational and educational purposes only and does not constitute medical advice. Product descriptions, protocols, or references are not intended to diagnose, treat, cure, or prevent any disease. Always consult a qualified healthcare provider before beginning any new protocol, supplement, or treatment.

By purchasing from this website, you acknowledge and agree to use these products responsibly and in accordance with all applicable laws and guidelines.