SS-31 (Elamipretide) is a mitochondria-targeted peptide studied for boosting cellular energy, reducing oxidative stress, and supporting heart, muscle, and neurological function. It became FDA-approved in 2025 for Barth syndrome.
Quick Facts
Reconstitution: Add 3.0 mL bacteriostatic water → ~16.67 mg/mL
Daily Range:5–10 mg/day (standard), 15–20 mg/day (advanced, supervised)
Easy Measuring: 1 unit = ~167 mcg on a U-100 insulin syringe
Storage: Freeze when dry; refrigerate after mixing; use within 4 weeks
Dosing & Reconstitution Guide
Standard Gradual Approach (3 mL = 16.67 mg/mL)
This concentration keeps standard doses in a manageable syringe volume.
Reconstitution Steps (Easy Version)
Draw 3.0 mL bacteriostatic water
Slowly drip down vial wall
Swirl gently (no shaking)
Refrigerate immediately; protect from light
Use within 4 weeks
Note: Educational use only; not medical advice.
Supplies Needed
SS-31 Vials
8 weeks (5–10 mg/day): ~10 vials
12 weeks (10 mg/day): ~16 vials
12 weeks (Advanced up to 15 mg/day): ~21 vials
Syringes
1 per day for standard dosing
2 per day only if splitting 20 mg advanced doses
Totals:
8 weeks → 56 syringes
12 weeks → 84 syringes
Bacteriostatic Water
8 weeks → 30 mL
12 weeks → 48 mL
12 weeks advanced → 63 mL
Alcohol Swabs
2 per day (vial + skin)
Totals:
8 weeks → 112
12 weeks → 168
Protocol Overview
Goal: Support mitochondrial ATP production and reduce oxidative stress
Schedule: Daily SC injections for 8–12 weeks
Standard Dose:5–10 mg/day
Advanced Dose:15–20 mg/day (requires splitting at 20 mg)
Storage:
Lyophilized → freeze
Reconstituted → fridge (use within 4 weeks)
Daily Dosing Protocol
Standard Approach
Weeks 1–2: Start 5 mg/day
Week 3+: Increase to 10 mg/day (most common trial dose)
Advanced Approach (Supervision Only)
Weeks 5+: 15–20 mg/day
20 mg requires two injections (split dose)
Timing
Once per day
Same time each day
Rotate injection sites
Cycle Length
8–12 weeks total
Limited long-term data past 12 weeks
Storage Instructions
Lyophilized
Store at −20 °C
Keep dry, avoid light
Reconstituted
Refrigerate immediately
Use within 4 weeks
Never refreeze
Let vial reach room temp before opening to avoid condensation
Important Notes
Use a new sterile syringe for every dose
Rotate injection sites (abdomen, thighs, upper arms)
Inject slowly to reduce irritation
Log doses, sites, and any reactions
Split doses only when >15 mg/day
How SS-31 Works
SS-31 is a mitochondria-targeted peptide that binds to cardiolipin, a key lipid that organizes the electron transport chain. By stabilizing this structure, SS-31 helps:
Improve ATP production
Reduce oxidative stress
Support cells under metabolic strain
Research shows protective effects in:
Heart and muscle function
Neurological stress
Mitochondrial disorders
Age-related fatigue and muscle decline
The TAZPOWER trial in Barth syndrome showed meaningful gains in strength and endurance, leading to FDA approval in 2025.
Potential Benefits & Side Effects
Potential Benefits
Improves mitochondrial efficiency
Enhances ATP output
Reduces oxidative stress
Strengthens muscle performance (Barth syndrome data)
Protective effects in multiple preclinical models
Common Side Effects
Mild skin irritation at injection site
Redness or itching (very common, usually mild)
No serious or dose-limiting side effects reported
Limited long-term safety data (>12 weeks)
Lifestyle Tips for Better Results
Eat mitochondrial-supportive nutrients (B-vitamins, magnesium, CoQ10, ALA)
Combine strength + cardio training
Aim for 7–9 hours of sleep nightly
Manage stress through breathwork, meditation, or light movement
Minimize “mitotoxins”: excess alcohol, environmental toxins, avoidable medications
Injection Technique
Clean vial + skin
Draw dose; remove air bubbles
Pinch skin; inject at 45–90°
Inject slowly over 3–5 seconds
Wait briefly; withdraw gently
Rotate sites (2+ inches apart)
Dispose needle in sharps container
Disclaimer:
All peptide products sold on this website are for research, laboratory, and educational purposes only. They are not approved by the FDA or Health Canada, are not intended for human consumption, and should not be used for medical, cosmetic, or therapeutic purposes unless specifically prescribed by a licensed healthcare professional.
The information provided on this site is for informational and educational purposes only and does not constitute medical advice. Product descriptions, protocols, or references are not intended to diagnose, treat, cure, or prevent any disease. Always consult a qualified healthcare provider before beginning any new protocol, supplement, or treatment.
By purchasing from this website, you acknowledge and agree to use these products responsibly and in accordance with all applicable laws and guidelines.