TB-500 is a synthetic peptide derived from thymosin beta-4, studied in preclinical research for its potential role in tissue repair, recovery signalling, and cellular migration processes. It is not FDA-approved for therapeutic use but remains a topic of interest in early research models.
Reconstitution
Add 5 mL bacteriostatic water → final concentration - 2mg/mL
Unit conversion:
Weekly dose reference: 2–5 mg weekly total.
Easy math: At 2 mg/mL,
👉 1 unit = 20 mcg on a U-100 insulin syringe
Storage:
Lyophilized powder: freeze at –20 °C (–4 °F)
After reconstitution: refrigerate at 2–8 °C (35.6–46.4 °F)
Do not freeze reconstituted solution
Weekly Dosing Schedule
TB-500 protocols are typically divided into:
• Loading Phase
• Maintenance Phase
Phase | Weekly Amount | Syringe Units | Volume |
Weeks 1–4 (Loading) | 2 mg per week | 100 units | 1.00 mL |
Weeks 5+ (Maintenance) | 2–5 mg per week | 100–250 units | 1.00–2.50 mL |
Frequency: Typically administered 1–2 times weekly
Reason for gradual increases: observational reports suggest tolerability may improve with conservative introduction.
*Split dosing or additional vials may be necessary. Weekly doses may be fractionated into twice-weekly administrations (e.g., 1mg twice weekly) to support tolerability or administration preferences.
Tip: Divide weekly dose into 2 injections (e.g., Monday + Thursday).
Reconstitution Steps
Draw 5 mL bacteriostatic water with a sterile syringe.
Inject slowly down the vial wall.
Swirl gently — do not shake.
Label with the date and concentration.
Refrigerate at 2–8 °C (protected from light).
Note: TB-500 is not FDA-approved for human use and is for research purposes only.
Supplies Needed
For an 8–16 week protocol:
TB-500 10mg Vials
8 weeks: 2-4 vials
12 weeks: 3-6 vials
16 weeks: 4-8 vials
Syringes (2 per week)
8 weeks: 16
12 weeks: 24
16 weeks: 32
Bacteriostatic Water
8 weeks: 10–20 mL
12 weeks: 15–30 mL
16 weeks: 20–40 mL
Alcohol Swabs
8 weeks: 32
12 weeks: 48
16 weeks: 64
Protocol Overview
A clean summary of the weekly research schedule:
Goal: Support tissue repair, cell migration, angiogenesis
Frequency: once weekly or biweekly subcutaneous injections
Dose Range: 2mg - 5mg per week
Cycle Length: 8–12 weeks, optional extension to 16 weeks
Reconstitution: 5.0 mL water per 10 mg vial for ideal accuracy
Storage: Powder frozen; solution refrigerated
Dosing Protocol
Loading phase:
1-2 mg weekly split into 2 injections.
Maintenance:
2-5 mg weekly.
Timing:
Spread injections evenly throughout week.
Tip: Consistent scheduling improves measurement accuracy.
Storage Guidelines
Keep your peptide stable and effective:
Lyophilized:
Store at –20 °C
Keep dry and protected from light
Reconstituted:
Store at 2–8 °C
Never freeze after mixing
Use within 28 days
Let the vial warm slightly before opening to prevent condensation.
Important Notes
Best-practice suggestions for safe, consistent research use:
Always use a new sterile syringe
Dispose of needles in a sharps container
Inject slowly to prevent leakage
Wait 5–10 seconds before withdrawing the needle
Document your dosing and observations
TB-500 is WADA-banned for athletes and not FDA-approved for humans
How TB-500 Works
TB-500 contains the active N-terminal fragment of thymosin beta-4 (Ac-LKKTETQ), the region responsible for:
Actin binding
Cell migration
Angiogenesis
Tissue regeneration
Animal studies show enhanced collagen deposition, improved wound healing speed, and reduced local inflammation. Some research also suggests TB-500 may act as a prodrug, converting into an even more active metabolite.
Potential Benefits & Side Effects
Insights from preclinical research:
Potential Benefits
Faster wound and tissue repair
Improved angiogenesis
Reduced inflammation
Enhanced cell migration
Possible Side Effects
Mild injection-site irritation
Limited human data; effects primarily studied in animals
Lifestyle Support
Factors that may support recovery in research models:
Prioritize recovery practices.
Avoid excessive physical strain during injury studies
Maintain consistent sleep
Support balanced stress levels
Injection Technique (Subcutaneous)
A quick refresher:
Clean vial and skin with alcohol; air dry.
Pinch a fold of skin.
Insert needle at 45–90 degrees.
Inject slowly and steadily.
Wait 5–10 seconds before withdrawing.
Rotate injection sites each day.
Disclaimer:
All peptide products sold on this website are for research, laboratory, and educational purposes only. They are not approved by the FDA or Health Canada, are not intended for human consumption, and should not be used for medical, cosmetic, or therapeutic purposes unless specifically prescribed by a licensed healthcare professional.
The information provided on this site is for informational and educational purposes only and does not constitute medical advice. Product descriptions, protocols, or references are not intended to diagnose, treat, cure, or prevent any disease. Always consult a qualified healthcare provider before beginning any new protocol, supplement, or treatment.
By purchasing from this website, you acknowledge and agree to use these products responsibly and in accordance with all applicable laws and guidelines.