All Collections
Advanced tips and tricks
Features To Help With Fluorochrome Selection
Features To Help With Fluorochrome Selection

spillover pop-ups, spectra viewer and % filter values

Matthew Camilleri avatar
Written by Matthew Camilleri
Updated over a week ago

FluoroFinder can not build an entire flow cytometry panel for you but several powerful features can help you quickly make better choices about what fluors to use in your panel design.  

FluoroFinder uses the term % Filter to describe how well emission from a particular fluor will be captured on your cytometer.  These values can be found by hovering your cursor over any fluorochrome on the Products page.  We define % Filter as the percentage of the area representing your filter set that is covered by the emission curve.

These % Filter pop-ups are intended to give you a quick idea of how well your flour can be detected in each channel on your cytometer.  The best part is that these pop-ups will also include a column for any other fluor you have already selected that impacts any of the same channels at 2% or greater.  This allows you to quickly see and avoid selections that will result in high spillover levels.

Another fantastic way to spot potential problems with your fluorochrome choices is to use the multi-vendor spectra viewer built in to the top of Panel Builder.  After you select one or more fluors on the Products page you will be able to see excitation and emission curves within the context of your preloaded cytometer configuration.  Use the toggle buttons to see your fluorochromes normalized to each laser line.

If you want to explore fluorochrome interactions in greater detail outside of the panel builder, try using our stand alone spectra viewer.

Did this answer your question?