The articles in this section explain how to generate manual instructions for scientists to follow in the lab using hand-held pipettes. To learn more about the Synthace functionality for automated miniaturized purification, click here.
Robocolumns (RBCs) are a form of miniaturized purification. As the name suggests, they have been developed for use with liquid handling automation (primarily on the Tecan Evo and Fluent devices). However, in the absence of access to liquid handling automation, or for the purposes of training, you may still want to build and iterate on RBCs workflows in Manual Execution mode.
In this article, we will provide an example RBCs workflow and show how you can simulate it in Manual Execution mode.
Get started
Read the introduction to this series of tutorials, then complete all of the previous tutorials in the series. For more information, click here.
Learn more about miniaturized purification workflows from the Tutorials
The 🎓 Get Started: Introduction to Miniaturized Purification tutorial is a great place to get started.
Optionally, to learn more about miniaturized purification and RBCs in Synthace, click here.
Simulate a miniaturized purification workflow in Manual Execution mode
Open the 🎓 Get Started: Introduction to Miniaturized Purification tutorial. You can find this by searching in the Tutorials section of the platform.
Open the Protein Purification with Fraction Collection example in the builder.
This workflow describes a simple miniaturized purification workflow.
It includes:a definition of the filter matrices;
the layout of the RBCs plate;
a definition of the buffers and samples;
steps for the equilibration, sample loading, washing and elution stages; and
a step for the plate reading of the samples.
Make sure that Manual Execution mode is selected and that you specify an input plate type for your various buffers or reagents.
Note: The input plate type must have a large enough volume capacity per well to contain all the required buffers for this workflow to simulate.Simulate the workflow by clicking Simulate in the top right hand corner of your screen, and navigate to the simulation details by clicking View Simulation in the Simulations panel.
Navigate to the Preview tab and click through the simulation to view the steps of this process.