The articles in this section explain how to generate manual instructions for scientists to follow in the lab using hand-held pipettes.
Not all experiments in the lab are run on liquid handling robots. You may not have access to your liquid handling device, you may want to perform some small-scale experiments to test your biology before you scale up to automation. Alternately, you might just want to do a manual experiment with no intention of automating it, but still want to design, plan and record your experiment in Synthace. Manual Execution mode can be used for these purposes and more.
Get started
Read the introduction to this series of tutorials. For more information, click here.
Create a workflow in the Builder. To learn how, click here.
Example: We have used a simple aliquot workflow as an example here. Manual Execution mode is selected by default.
Simulate in Manual Execution mode
If you have not used an instance of the Define Liquids and Plates element to define a specific plate of source liquids, specify the input plate type from which you want to aliquot your source liquids, to learn how click here.
Once you have created your workflow and specified your input plate type (if required), click Simulate.
Preview the simulation
After you simulate the workflow, click View Simulation to open the simulation details.
Open the Preview tab, then click through the steps to check that the instructions that Synthace has generated are correct.
In the aliquot example used, you will see 16 aliquots of Liquid A, 4 aliquots of Liquid B and 1 aliquot of Liquid C made to the same plate. Synthace groups identical transfer instructions by column, so for this example you should see 4 steps in your protocol, one for each column.
Download your manual instructions
Click Start Execution.
Open your manual instructions, by clicking Read Instructions.
Continue to Tutorial 1.2 to learn how to interpret the manual instructions.