In Synthace you are able to generate a simulation of your workflow showing all the liquid transfers that will take place to carry out your protocol, whether that be manually or with liquid handling automation.

However, when you are doing a DOE it may not be possible to set up all the runs from your design in a single execution. Therefore, Synthace lets you split you the runs from your design into multiple simulations and subsequent executions. These simulations are what we now call a Simulation Series.

Simulations in a series are related to one another by the DOE design that generated them, with each simulation containing a subset of the runs from your design. Only when all simulations from the series have been executed in the lab will you have collected the data for your entire DOE design.

With most liquid dispensers you are limited to only having a single output plate position. In some cases, you might need to use a 96 well plate for your assay but you have 384 runs in your DOE design. In this situation, Synthace can automatically generate 4 simulations as part of a series, where each simulation will provide the device instructions required to set up 96 of the required runs.

Some factors, often hard to change factors, require groups of runs to be allocated to specific plates, where all the runs on a plate have the same level of that factor. A good example of this is temperature. If you are investigating temperature as a factor it is very difficult and often not possible to control the temperature on a well per well basis of a microtiter plate. Usually an entire plate is placed into an incubator where all the wells will be at the same set temperature. Therefore, Synthace allows you to split the runs from your DOE design to different simulations based on Factors and their levels. Each simulation of this series would therefore contain the runs that will be incubated at a specific temperature.