There are often constraints in the lab that will require you to divide the runs in your DOE design into groups to facilitate executing your experiment. These can include having more runs that can fit into a single plate, but the liquid handling device you are using only supports a single plate at a time, or you have hard to change factors that need all the runs at one level on one plate and all the runs at another level on a separate plate.
In this article you will learn:
how to group runs from your DOE design to separate simulations based on factors and their levels.
Getting started
Grouping runs by a single factor and its levels
Scroll to the bottom of the DOE Design panel and click on the Add button under Group runs by.
Select the factor from the drop down that you want to group runs by.
Synthace will tell you how many simulations will be required to satisfy the run grouping.
Scroll in the design matrix table to see which runs have been grouped to a simulation. In this example, all of the runs with factor Liquid B at 20 mM have been grouped to Simulation 1 and all those with factor Liquid B at 200 mM have been grouped to Simulation 2.
Grouping runs by a multiple factors and their levels
Continuing from above, click on the Add button under Group runs by to add a second factor.
Select a second factor from the drop down that you want to further group runs by.
Synthace will tell you how many simulations will be required to satisfy the run grouping.
Note: In this situation 4 simulations are required. Grouping runs by two factors both with 2 levels each results in 4 unique groups.
Scroll in the design matrix table to see which runs have been grouped to a simulation. In this example, all of the runs with factors Liquid B at 20 mM and Liquid C at 50 mM have been grouped to Simulation 1, all those with Liquid B at 200 mM and Liquid C at 50 mM have ben grouped to Simulation 2, all those with Liquid B at 20 mM and Liquid C at 200mM have been grouped to Simulation 3 and all those with Liquid B and C at 200mM have been grouped to Simulation 4.
Simulating series
Once you have completed your grouping, click on Simulate with design.
Note: All simulations will be started simultaneously
Well done for making it to the end of this tutorial.
To learn how to group runs based on run capacity, click here.
To learn about data structuring and analysis in Synthace, click here.