No, standard EPUB validation tools typically do not check for accessibility requirements mandated by EU laws or other accessibility standards. These tools focus on the technical correctness of the EPUB format itself.
Accessibility-specific checks are handled by dedicated tools like the ACE Accessibility Checker, which provides a detailed accessibility compliance report.
You can access ACE here: https://daisy.org/activities/software/ace/
Currently, it is expected that EPUB validators will evolve in the future to better integrate accessibility compliance checks alongside technical validation.