A Single Ingredient material contains only one component (i.e. DMEM-F12) and a Recipe Material usually contains more than one Single Ingredient material (i.e., hESC Complete Medium) and requires some preparation prior to usage.
Before building Recipes, we recommend you add the Single Ingredient Materials or perform a bulk single ingredient upload of the materials you will use as ingredients in your recipe first (see, Add Single Ingredient Materials or Import Single Ingredient Materials ) and then build Recipe Materials following the workflow below.
Adding Recipe Materials
From the CultureTrax Dashboard, click 'Materials'.
From the 'Find Materials' screen, select the "Add a Material" button.
What kind of Material is it?
3. Select 'Recipe' (a Recipe Material usually contains more than one Single Ingredient material (i.e., hESC medium) and requires some preparation prior to usage.)
4. The Material Name is a required field. (This name does not need to be unique, but it is good practice to check if the material already exists with a slightly different name (i.e., check if Essential 8 is already added as E8 medium)
5. Select a Storage Temperature
6. Set the Ending Concentration
7. Click 'Add Ingredient to this Recipe' button and you are taken back to the Material Library where you can select any Single Ingredient or Recipe Material.
Choose a Material
8. You can use the Material Type filters at the left or the search box to find your first ingredient.
9. Click 'Configure Recipe Ingredient' to select the material you want to use in your Recipe.
10. Enter the Starting Concentration of the ingredient.
11. Ending Concentration, or the amount used of the Ingredient you are configuring for the Recipe and their corresponding units from the drop-down selection. (Note: Units are required if you enter a value to the left)
12. Click 'Add Ingredient to Recipe' and the information will populate to the 'Ingredients' section of your recipe where you can Edit the configuration.
Continue adding all of the ingredients until your Recipe is complete.
13. Prep Procedure
Recipe Materials typically have a Prep Procedure. You can copy and paste directly into the Prep Procedure section of the rich text editor from your Recipe Material's method/protocol/user guide . The Prep Procedure will always be available to reference when you access the Recipe Material in CultureTrax. Use the toolbar buttons to format your Prep Procedure as you like.
Select Material Attribute(s) (required)
14. Selecting a Material Type from the drop-down list is like a 'tag' that can help you quickly find it later. Some material types have a hierarchy and if you select the sub-type (i.e., Serum-free) your material will automatically be tagged with both the material type and sub-type. (i.e., Complete Media, Serum-free). CultureTrax uses Material Types to help you populate Protocol Templates and you can filter by material type from the left panel of the Find Materials page.
15. Add to Collection
If you have an Admin account status you will have the choice of sharing the material with other lab members (the material will be tagged with 'Lab') or keeping it designated for personal use. If you have a User account status you do not have this option and all materials you create will be for Personal use only. (Note: any materials you create as a User can be edited later for 'Lab' sharing if your account status is changed to Admin. Your Lab's 'Primary Contact' can edit your account status - see Adding and Managing Lab Members
16. Other Material Attributes
Select Xeno-free, Aliquots or Stock as an optional tag for you material.
17. Add Documents
Adding a document to your material is optional. When you click the 'Add Document' button you will be taken to a 'Select a Document' screen to find the one you want or you can Upload a New Document.
Create Material or Save Draft
18. Click 'Create Material' and you're done! Your new Recipe Material will appear in your Materials library. (If you want to finish later, you can click 'Save Draft' and your Recipe Material will not be shared with other lab members until you 'Edit' and 'Save' the draft version.)