Best Practices for Materials

Learn the best practice for adding materials that require dilution or aliquoting

Kevin Conard avatar
Written by Kevin Conard
Updated over a week ago

For Single Ingredient materials that require some aliquoting or dilution, (such as A14700 or Vitronectin) we recommend you first add the stock (lyophilized or concentrated) material as a Single Ingredient material (i.e., A14700 - 60ul)

You can then build Recipe Materials for the working aliquots that include the concentration/size of the aliquot in the material name.

For example:  To add Vitronectin and Vitronectin-coated plates as materials in CultureTrax, add the Vitronectin stock vial as a Single Ingredient and add a Recipe Material for the smaller Vitronectin aliquots. You could then add a Recipe Material for coated plates from the aliquots.

  1.  Add the Vitronectin stock as a new Single Ingredient material named "Vitronectin 0.5 mg/ml Stock"

  2. Add/Build a new Recipe Material named "Vitronectin - 60 ul aliquots" by adding the ingredient "Vitronectin 0.5 mg/ml Stock" (#1 above) and describing the aliquoting procedure and volumes.

  3. Add/Build a new Recipe Material named "Vitronectin coated 6-well plates" by combining the Recipe Material "Vitronectin aliquots" (#2 above) with the additional Single Ingredient materials "Cell Culture Plates, 6-well, Flat Bottom (Costar)" and "DBPS, no calcium, no magnesium", describing your volumes and plate coating procedure.

For example:  To add a Rock Inhibitor (Y27632) as a material in CultureTrax, you would add the lyophilized stock as a Single Ingredient and the working aliquots as Recipe Material.

  1. Add the stock Rock Inhibitor material as a Single Ingredient material named "Y27632 -  1mg stock".

  2. Add DPBS (the diluent) as a Single Ingredient material 

  3. Add/Build a Recipe Material named "Y27632 - 5mM Aliquots" by combining the Y27632 - 1mg stock (#1 above) with DPBS (#2 above), describing the volumes, concentrations, prep procedure and storage location of the aliquots here.

Did this answer your question?