Welcome to Proofig AI's tutorial on best practices and tips for analyzing Western blot images. This guide will provide you with the recommended tools and techniques to ensure accurate and thorough image analysis.

For analyzing Western blot images, we recommend using the Emboss filter and Histogram Equalization enhancement in Proofig. These tools can highlight different aspects of the image and improve overall visibility.

Let's see how the Emboss filter can be applied to a Western blot image to highlight suspected reuse issues.

Here's an example of a Western blot image where reuse is suspected.
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Applying the Emboss filter highlights certain features in the background, making it easier to identify and confirm the reuse of the image. These types of tools are particularly relevant for identifying duplication and reuse within scientific images. To learn more, visit our page on duplication and reuse detection.

Here's an example of a Western blot image with a suspected deleted band. The system marks the suspected area with a small red indicator.
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Now, let’s see how Histogram Equalization can enhance the image to uncover this suspected deletion. Applying Histogram Equalization adjusts the contrast, redistributing the pixel intensity values to span the entire range. This enhancement makes the deleted band more visible, helping to confirm the manipulation. These techniques are especially useful for identifying alterations and manipulations in Western blot images. To learn more, visit our page on alteration and manipulation detection.

Finally, here's an example of a Western blot image with a suspected splice. The system marks the suspected area with a small red indicator.
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Let's see how the Emboss filter can help detect a spliced Western blot image.
Applying the Emboss filter creates a 3D effect by highlighting the edges, making it easier to identify the splice and confirm the manipulation.

Using the right tools and techniques is essential for accurate Western blot image analysis. For a more detailed explanation of our detection tools, what they are, and how they work, please refer to our 'Key Detection Filters and Tools' article. Thank you for using Proofig.