If you select Let Synthace Optimize, Synthace will allocate the dilutions and their replicates in the most efficient way it can. If you select this, you must populate the PlateSetNames and PlateTypes inputs.
If you select Replicates Together and Dilutions Apart, Synthace will allocate the replicates of the same dilution to adjacent wells, but keep the dilutions of the same liquid apart.
Example
Let’s say that you want to make eight dilutions of three liquids (green, blue and yellow), and four replicates of each dilution. If you select this option, Synthace will allocate:
every replicate of the first dilution of green to A1:D1;
every replicate of the first dilution of blue to E1:H1;
every replicate of the first dilution of yellow to A2:D2;
every replicate of the second dilution of green to E2:H2;
every replicate of the second dilution of blue to A3:D3; then
every replicate of the second dilution of yellow to E3:H3, and so on.
If you select Replicates Apart and Dilutions Together, Synthace will allocate the dilutions of the same liquid to adjacent wells, but keep the replicates of the same dilution apart.
Example
Let’s say that you want to make eight dilutions of three liquids (green, blue and yellow), and four replicates of each dilution. If you select this option, Synthace will allocate:
the first replicate of every dilution of green to A1:H1;
the second replicate of every dilution of green to A2:H2, and so on;
the first replicate of every dilution of blue to A5:H5;
the second replicate of every dilution of blue to A6:H6, and so on;
the first replicate of every dilution of yellow to A9:H9; then
the second replicate of every dilution of yellow to A10:H10, and so on.
If you select Replicates And Dilutions Together, Synthace will allocate the replicates of the same dilution and the dilutions of the same liquid to adjacent wells.
Example
Let’s say that you want to make eight dilutions of three liquids (green, blue and yellow), and four replicates of each dilution. If you select this option, Synthace will allocate:
every replicate of the first dilution of green to A1:D1;
every replicate of the second dilution of green to E1:H1, and so on;
every replicate of the first dilution of blue to A5:D5;
every replicate of the second dilution of blue to E5:H5, and so on;
every replicate of the first dilution of yellow to A9:D9; then
every replicate of the second dilution of yellow to E9:H9, and so on.
If you select Replicates And Dilutions Apart, Synthace will keep the replicates of the same dilution and the dilutions of the same liquid apart.
Example
Let’s say that you want to make eight dilutions of three liquids (green, blue and yellow), and four replicates of each dilution. If you select this option, Synthace will allocate:
the first replicate of every dilution of green to A1:H1;
the first replicate of every dilution of blue to A2:H2;
the first replicate of every dilution of yellow to A3:H3;
the second replicate of every dilution of green to A4:H4;
the second replicate of every dilution of blue to A5:H5; then
the second replicate of every dilution of yellow to A6:H6, and so on.
