In this tutorial we will cover how you can build up more complex workflows from smaller workflow units.
Often you may have a set of samples of varying concentrations where some of those samples have a concentration too high for the downstream application. As such you may want to normalize only those samples that are above your concentration threshold. Here we will cover how you are able to do this by combining two smaller workflow units.
Get started
Read the introduction to this series of tutorials. For more information, click here.
Open the workflow that you created in Core elements tutorial 4.1.
In this workflow a set of DNA samples of varying concentrations were all normalized to 20 ng/ul in a new plate.
Open the workflow that you created in Core elements tutorial 8.1.
In this workflow liquids with a concentration above a threshold of 25 uM were selected from a set of liquids and aliquot to a new plate.
Normalize a selection of liquids whose concentrations are above a threshold
Lets pull these two concepts together to select a set of liquids above a specific threshold to be normalized to a new plate.
Create a copy of the workflow that you created in Core elements tutorial 8.2. To learn how, click here.
Rename the copy Core elements tutorial 9.1. To learn how, click here.
Add an extra Select element to the workflow canvas.
Delete the connection between the Define Plasmid DNA and Normalize elements.
Connect the Liquids output parameter from your Define Plasmid DNA element to the Liquids input parameter of your Select element. Then connect the Selected Liquids output parameter from the Select element into the Liquids To Normalize input parameter of your Normalize element.
In the Select element specify that you want to select all liquids whose concentration is greater than 30 ng/ul.
Simulate the workflow
Check that the device that you selected can follow the instructions that you prepared. To learn how, click here.
Preview the execution
After you simulate the workflow, click View Simulation to open the simulation details.
Open the Preview tab, then click through the steps to check that the instructions that Synthace has generated are correct.
Notice that now only those liquids whose concentrations were greater than 30 ng/ul have been normalized to a new plate in duplicate rather than all liquids.
Check your work:
To see what your finished workflow to this tutorial should look like simply navigate to the Tutorial and search for Tutorial: 9. Combining small workflow units.
