Cherry picking samples from a larger set that meet a specific physical property, such as concentration, is often a familiar task to scientists in the laboratory. Often there can be downstream experiments or analytics that require samples to have a concentration greater than a certain threshold to be used. Therefore, cherry picking all samples that have a concentration greater than the threshold quickly and efficiently to a new piece of labware can be extremely useful.
Get started
Read the introduction to this series of tutorials, then complete all of the previous tutorials in the series. For more information, click here.
Create a workflow in the builder. To learn how, click here.
Rename the workflow Core elements tutorial 8.1. To learn how, click here.
Select the device on which you want to run your workflow. To learn how, click here.
Select some samples by concentration
If you have a set of samples of varying concentration and you want to select just those samples with a concentration above a threshold and aliquot those samples to a new plate, try using the Select and Aliquot elements.
In the Workflow Builder add three elements to your canvas: 1 x Define Liquids And Plates, 1 x Select and 1 x Aliquot.
Connect the Output Liquids parameter from your Define Liquids And Plates element into the Liquids input parameter of your Select element.
Connect the Selected Liquids output parameter from your Select element into the Liquids To Aliquot input parameter of your Aliquot element.
Define your Samples that are intended for normalization in the Define Liquids And Plates element.
Here we have used the Table UI to define all of our samples, importantly with their concentrations.
In the Select element select the
Alloption in the Condition parameter.Specify the concentration threshold above which you want to select your samples by choosing the
Greater Thancomparison and your concentration threshold, here 25 uM, in the Concentration parameter.
Aliquot the samples
Specify aliquot parameter values in the Aliquot element for the aliquot volume, number of replicates and the Layout options for the Aliquot plate.
Simulate the workflow
Check that the device that you selected can follow the instructions that you prepared. To learn how, click here.
Preview the execution
After you simulate the workflow, click View Simulation to open the simulation details.
Open the Preview tab, then click through the steps to check that the instructions that Synthace has generated are correct.
In this example twelve of the samples have concentrations greater than the threshold and are cherry picked from PlateA containing our source samples to PlateB.
Check your work:
To see what your finished workflow to this tutorial should look like simply navigate to the Tutorials and search for Tutorial: 8. Select.
