Devices
A Tecan Freedom EVO® or Tecan FLUENT® .
A Te-Chrom®.
A Te-Shuttle.
A Te-Chrom waste pump.
A plate reader.
Labware
Some long or short robocolumns.
Note: Although you must use either long or short robocolumns (not both), each one can contain a different volume of resin
A robocolumns array plate.
Fixed tips on your liquid handler’s FCA.
Some collection plates, to collect fractions.
One or more input plates, to contain the samples and buffers at the start of the execution.
Example: A 100 mL trough, a 12-well trough, or a tube.
Liquids
The protein samples you want to purify.
One or more buffers for each chromatography stage.
Example: A buffer for the equilibration stage, a buffer for the wash stage, a buffer for the elution stage, etc.
Tip: You can use the same buffer for different chromatography stages.