A mixture that contains one or more protein(s) of interest and some contaminants. To purify a sample, a resin separates the protein of interest from the contaminants (or vice-versa).

In a sample, the components that you want to discard.

Each robocolumn contains a resin. When a Tecan Freedom EVO® or Tecan FLUENT® dispenses a sample into a robocolumn, the resin separates the protein of interest from the contaminants ( or vice-versa): one binds to the resin for you to keep, and one passes through for you to throw away.

A miniaturized purification column that sits in a well on a robocolumn array plate. A long robocolumn contains up to 600μl of resin. A short robocolumn contains up to 200μl of resin.

An array plate with wells that contain either long or short robocolumns. On the deck of a Tecan Freedom EVO® or Tecan FLUENT®, the robocolumn array plate sits on the Te-Chrom®.

A device that holds the robocolumn plate and the waste tray.

A device that clips on top of the robocolumn plate to hold the individual columns in place.

A plate that collects the fractions that pass through the robocolumns. On the deck of a Tecan Freedom EVO®, a collection plate sits on the Te-Shuttle.

During each chromatography stage in which you collect fractions, the robocolumn plate remains in situ, and the Te-Shuttle moves the collection plate. This means that different columns of the collection plate are beneath the robocolumns, ready to collect different fractions.

The liquid that you collect from a robocolumn in a single well or send to the waste tray. For each chromatography stage, you specify the number of fractions that you want to collect or send to the waste tray. The volume of each fraction is a multiple of the capacity of the robocolumns that you use. This means that the volume of liquid that you collect or send to the waste tray can be greater than the capacity of the robocolumn through which it passes.