The protocol uses a column chromatography method to isolate a protein of interest.
In a series of chromatography stages, a liquid handler dispenses a sample and some buffers into a number of robocolumns, and the robocolumns separate the protein of interest from any contaminants in the sample (or vice-versa).
You select the fractions that you want to send to a plate reader, and the plate reader measures their absorbance.
Synthace normalizes and structures the data from the plate reader, matches each fraction to the chromatography stage during which you collected it, and calculates the mass, mass concentration and yield of the protein of interest.
It presents these results in an interactive visualization.
Together, the automation of the protocol and the visualization of the results make it quick and easy to develop, optimize and execute your purification process.
Summary
To automate a miniaturized purification protocol that uses robocolumns, complete the following steps.