Using Custom Liquid Policies on your Tecan Fluent®

For a general overview of using liquid policies with Synthace, click here.

For information on how to use a custom liquid policy spreadsheet, click here.

For information on how to add a liquid handling policy to an element, click here.

Specifies where in the container aspiration will occur. Appropriate input values are bottom, or liquid. For LLD and LLF, the aspiration zone must be set to liquid. Specifying bottom means Synthace will aspirate from the Z-Max definition in Fluent Control.

Turns on liquid level detection during aspiration. Appropriate inputs are on, off, or auto. If Synthace determines that liquid volume is too low from the input container, LLD will automatically be overridden and Synthace will instead aspirate from the bottom of the well when using auto.

Turns on liquid level following during aspiration. Appropriate inputs are on, off, or auto. If Synthace determines that liquid volume is too low from the input container, LLD will automatically be overridden and Synthace will instead aspirate from the bottom of the well when using auto.

Specifies where in the container dispense will occur. Appropriate input values are bottom, top or liquid. For LLD and LLF, the dispense zone must be set to liquid. Specifying top means Synthace will dispense from the Z-Dispense definition in Fluent Control and specifying bottom means Synthace will dispense from the Z-Max definition in Fluent Control.

Turns on liquid level detection during dispensing. Appropriate inputs are on, off, or auto. Note that to properly use LLD on dispensing, the container you are dispensing to must have liquid present. If Synthace determines that liquid volume is too low from the input container, LLD will automatically be overridden and Synthace will instead aspirate from the bottom of the well when using auto.

Turns on liquid level following during dispensing. Appropriate inputs are on, off, or auto. If Synthace determines that liquid volume is too low from the input container, LLD will automatically be overridden and Synthace will instead aspirate from the bottom of the well when using auto.

Specifies number of mixing cycles prior to aspiration. Appropriate inputs are positive integer values (0, 1, 5 etc.). Setting 0 as cycle number disables mixing.

Specifies where in the container pre-mixing will occur. Appropriate input values are bottom, or liquid. Specifying liquid means Synthace will mix from the liquid level and specifying bottom means Synthace will mix from the Z-Max definition in Fluent Control.

Proxy liquid to set when mixing before aspirating. Choose one of the following that best represents the fluid mechanical properties you are working with, either Serum, Aqueous, FastAqueous, Surfactant, Glycerol, Ethanol, DMSO.

Specifies number of mixing cycles after dispensing. Appropriate inputs are positive integer values (0, 1, 5 etc.). Setting 0 as cycle number to disables mixing.

Specifies where in the container where post-mixing will occur. Appropriate input values are bottom, or liquid. Specifying liquid means Synthace will mix from the liquid level and specifying bottom means Synthace will mix from the Z-Max definition in Fluent Control.

Proxy liquid to set when mixing after dispensing. Choose one of the following that best represents the fluid mechanical properties you are working with, either Serum, Aqueous, Surfactant, Glycerol, Ethanol, DMSO.

Proxy liquid to use for the transfer. Choose one of the following that best represents the fluid mechanical properties you are working with, either Serum, Aqueous, Surfactant, Glycerol, Ethanol, DMSO.

Number of times that tips will be re-used before being discarded. Clean tips will always be used when changing liquid type or if tips are contaminated by liquid handling actions. Appropriate inputs are positive integer values (0, 1, 5 etc.). Setting 0 as Tip Reuse Limit means new tips will always be used for every transfer.

Specifies the allowed tip types for a given liquid policy. Appropriate inputs are “FCA, 10ul SBS”, “FCA, 50ul SBS”, “FCA, 200ul SBS”, “FCA, 350ul SBS”, “FCA, 1000ul SBS” for unfiltered disposable tips or “FCA, 10ul Filtered SBS”, “FCA, 50ul Filtered SBS”, “FCA, 200ul Filtered SBS”, “FCA, 350ul Filtered SBS”, “FCA, 1000ul Filtered SBS” for filtered disposable tips. Be sure to include quotation marks (“) at the beginning and end of each input. Multiple tip types can be inputs as long as they are separated by a comma (eg. the input “FCA, 10ul SBS”, “FCA, 50ul SBS” would tell Synthace 10uL and 50uL tips are available for use in this policy).

Number of multi-dispenses that may be made. Appropriate inputs are positive integer values (0, 1, 5 etc.). Setting 0 as Multi Dispense Limit means unlimited multi dispenses. Multi dispenses are not counted as tip reuse. All transfers must have the same liquid type, and tip contaminating behaviors like post-mixing must be disabled.

Enables multichanneling. Appropriate inputs are true or false.

Allows selection of a specific liquid class. All policy parameters will be ignored when calling device specific liquid class. The liquid class must exist in Fluent Control and the name must match exactly.The specified class will be used for both aspiration and dispense steps in a given liquid transfer.

Volume of air to blowout after dispensing. Appropriate inputs are volume amounts (30 ul, 100 ul etc.).

Extra volume to aspirate from the source and discard with the tip after transferring, thus increasing the accuracy of the final dispense. If Tip reuse is enabled, it may be dispensed back to the source and re-aspirated. Appropriate inputs are volume amounts such as 30 ul, 2 ml etc. When multi dispensing, it is recommended to use a header volume.

Extra volume to aspirate from source and immediately dispense, can increase accuracy of the first dispense. Appropriate inputs are volume amounts such as 30 ul, 2 ml etc. When multi dispensing, it is recommended to use a prewet volume.