Synthace needs to know where the devices and carriers are, which tip types and microplates are on the device, and which liquid classes you have added. To tell Synthace this, your FAS added a Tecan Freedom Fluent® configuration to Synthace during its deployment to your organization.

If you need to change your deck layout, add new labware or update your liquid classes for the miniaturized purification protocol, you must add a new Tecan FLUENT® configuration to Synthace.

The worktable you wish to use with Synthace must contain the following:

Note: You might set up the deck layout of your Tecan FLUENT® differently, but it still requires this hardware.

Note: The worktable must have individual carrier definitions for each of these TeChrom items. If any are combined into one definition or if you have questions, please contact your FAS.

Example: Note that in the below screenshot there are selectable definitions for both the TeChrom Tower and TeChrom Waste Tray.
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The worktable you wish to use with Synthace may contain the following non-essential carrier:

If you wish to switch between collecting fractions in both deep and shallow well plates, click here.​

During a chromatography stage, if you do not want to collect the liquid that passes through the robocolumns, the Tecan Freedom FLUENT® will send the liquid to the waste tray instead.

Ensure that engage and disengage vectors are taught for the Techrom Waste Tray. Right click on the waste tray click Edit > Robot Vectors and check the vectors that are taught.

Note: The vectors must have the name “engage” and “disengage” included.

Miniaturized purification workflows use fixed tips. Ensure that in Fluent Control there is an FCA that is configured for fixed or “Standard” tips.

Note: The device can contain multiple FCAs (one for fixed tips and one for disposable tips). Synthace will always select the FCA with fixed tips for miniaturized purification workflows.
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Make sure to match labware between Fluent Control and Synthace. To learn more on how to match labware between Fluent Control and Synthace click here. To make sure that your robocolumns are configured correctly, click here.

When deploying the FLUENT®, your FAS will upload liquid classes into your device’s Fluent Control. Ensure that SYNTHACE Robo Single and SYNTHACE Robo Multi are available liquid classes.

If they are missing, contact your FAS.

In order to execute miniaturized purification, the Tecan Fluent uses the TeChrom pump and TeChrom Shuttle Transport. The pump is used to remove waste liquid from the waste tray following the dispense of uncollected fractions onto the robocolumns. The shuttle is used to position collection plates under the TeChrom to allow for collection of fractions.

In order for Synthace to communicate with these devices it is essential that both drivers are installed via the Pegasus Driver Framework as shown below. The naming of these driver is important to Synthace. Both drivers should be named appropriately, typically they are called “TeChromShuttle” and “Pump” respectively. The naming is defined by Tecan on device delivery, but can be modified if need. Check with you Customer Success team if you are unsure, or if your encounter errors during configuration generation.

Synthace needs to know which absorbance protocol you want the plate reader to use to read the collection plates.

To tell Synthace this, your FAS added a plate reader configuration to Synthace during its deployment to your organization.

If you want to use a different absorbance protocol for the miniaturized purification protocol, you must add a new plate reader configuration to Synthace. To learn more about how to add a new configuration, click here.