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Cyclops-7F Calibration

Cyclops-7F calibration procedure. Cyclops 7f calibration solution dyes.

Updated over a week ago

Hello there! Thank you for being our valued customer. We hope our product will meet your expectations.

To help you get started with using the Cyclops-7F for the first time or just to provide you with a refresher course on how to use this instrument, I will provide you with some quick links below that will have more in depth explanations.

Our document on recommended proxies for calibration using liquid dye standards can be found here:

Turner Designs Cyclops-7 Submersible Sensors
https://docs.turnerdesigns.com/t2/doc/tech-notes/S-0243.pdf
If unable to open the PDF, use this: Calibration Solutions for Various Instruments | Turner Designs Help Center

Our user manual on how to run a calibration can be found here:
998-2100 Rev 4.0 (turnerdesigns.com)
https://docs.turnerdesigns.com/t2/doc/manuals/998-2100.pdf

This is a helpful tip guide on setting up a calibration station for our Cyclops-7F:

Submersible Fluorometer Best Measurement Practices | Turner Designs Help Center

You may notice that not all calibration dyes are offered by our company. It is recommended that you purchase these from external sources that are recommended on the dye calibration sheet or find close alternatives. The main detail with these calibration solutions with the exception of Fluorescein and Rhodamine are that these are approximate calibrations. For a solution whose fluorescent response is similar to the fluorescent response of the actual fluorescent material you are studying, it is always better to use the real thing when able to.

As an illustrative example, we use Rhodamine WT at a concentration of 400 parts per billion (ppb), which produces a fluorescent signal comparable to that of 40 micrograms per liter (µg/L) of chlorophyll in vivo when measured with the Cyclops-7F fluorometer. During calibration, this fluorescent response would be assigned a value of 40 µg/L by the sonde or connected data logger.

It is important to note, however, that this calibration standard may not accurately reflect the actual chlorophyll concentration in your specific water system. Fluorescent responses vary among different chlorophyll-containing species, and the equivalence between Rhodamine WT and chlorophyll fluorescence is an approximation. For more precise monitoring, calibration using natural samples or species-specific standards may be necessary.

Dyes we sell

Rhodamine WT dye for calibration

  1. Chl in vivo (blue excitation)

  2. Phycocyanin (PC)

  3. Phycoerythrin (PE)

PTSA Dye for calibration

  1. Crude Oil

  2. CDOM

Solutions we do not sell

Turbidity Standards from AMCO

  • Turbidity standards recommended at 10, 100, and 1000 NTU. You can select any of these and more based on your application

Tryptophan Standard from Sigma Aldrich

  • Can be purchased as CAS No. 73-22-3

Refined Fuels Standards

  • 4500 NDSA is equivalent to 1 ppm BTEX. Ensure that it is CAS No. 76758-30-0
    1,5-Naphthalenedisulfonic acid disodium salt, dihydrate

Optical Brighteners

  • Any optical brightener can be used since this is a wide spectrum that is categorized under this umbrella term. You may use something similar to Sigma Aldrich's CAS No. 4404-43-7 or produce your own calibration standards from Tide or other laundry detergents.

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