FluoroSense
FluoroSense User Manual: 998-2852.pdf (turnerdesigns.com)
Name of Instrument | Instrument Part Number | Turner Designs Part Number for Calibration Solutions | Part Description |
FluoroSense CHLOROPHYLL | 2860-000-C | 6500-220 | FLUOROSENSE - CHLOROPHYLL uses RHODAMINE WT DYE, 800 PPB, 1 LITER |
FluoroSense PHYCOCYANIN | 2860-000-P | 6500-020 | FLUOROSENSE - PC, PHYCOCYANIN uses RHODAMINE WT DYE, 200 PPB, 1 LITER |
Cyclops-7F, C3, or C6P
Cyclops-7F, C3, and C6p Calibration Guide: Calibration guide.pdf
Cyclops-7F User Manual: 998-2100 Rev 4.0 (turnerdesigns.com)
C3 and C6p User Manuals: 998-2300.pdf (turnerdesigns.com)
Cyclops-7F, C3, or C6P Application Optics | Liquid Calibration Standard Concentration | Turner Designs Part Number for Calibration Solutions | Equivalent Fluorophore Concentration | Notes |
Chl in vivo (Blue) | Rhodamine WT 400 ppb | 6500-120 | 40 ug/L | Use our guide on our website: Turner Designs Cyclops-7 Submersible Sensors |
Chl in vivo (Red) | N/A | N/A | N/A | Use of known concentration primary standards is preferred |
Phycocyanin (PC) | Rhodamine WT 200 ppb | 6500-020 | 260 ppb | Use our guide on our website: Turner Designs Cyclops-7 Submersible Sensors |
Phycoerythrin (PE) | Rhodamine WT 50 ppb | 6500-020 | 500 ppb | Diluted from 6500-020 to 50 ppb (1 part dye to 3 parts deionized water) before use with Calibration Instructions 1.3 for PE units. |
Crude Oil | PTSA 100 ppb | 10-608 | 275 ppm | Osberg Light Crude Oil |
CDOM | PTSA 400 ppb | 10-609 | 125 ppb | Use our guide on our website: Turner Designs Cyclops-7 Submersible Sensors |
Turbidity | 10 NTU, 100 NTU, or 1000 NTU | Sold by AMCO Clear Standards purchased from GFS Chemicals |
| Sold by AMCO Clear Standards purchased from GFS Chemicals |
Tryptophan | 5000 ppb | Sold by Sigma Aldrich, CAS NO. 73-22-3 |
| Weigh out 0.1g of tryptophan into 1L ultrapure water to make 100mg/L solution (100,000ppb). Then, 50mL of the 100mg/L is transferred to another 1L volumetric flask and made up with ultrapure water to get 5000ug/L (5000ppb). |
Refined Fuels | 4500 ppb NDSA | CAS NO. 1655-29-4 | 1 ppm BTEX | Use our guide on our website: Turner Designs Cyclops-7 Submersible Sensors |
Optical Brighteners |
| Example: Sold from Sigma Aldrich |
| Can use any material under the optical brightener type. TIDE detergent is one example. |
AquaFluor
Use our user manual and calibration guide: Aquafluor Handheld Fluorometer (turnerdesigns.com) & Turner Designs AquaFluor Handheld Fluorometer Calibration Guide
AquaFluor Application Optics | Liquid Calibration Standard Concentration | Turner Designs Part Number for Calibration Solutions | Equivalent Fluorophore Concentration | Notes |
Ammonium |
| Not Applicable |
| Use our guide on website: Fluorometer Parameters | Ammonium | Turner Designs |
CDOM |
| Not Applicable |
| Calibrate using Quinine Sulfate or known concentrations of CDOM. Refer to website for example procedures: Fluorometer Parameters | CDOM | Turner Designs |
Chl in vivo | Rhodamine WT 400 ppb | 6500-120 | 60 ug/L | Use our guide on our website: Turner Designs AquaFluor Handheld Fluorometer |
Crude Oil | PTSA 100 ppb | 10-608 | 145 ppm | Oil Emulsions in water |
Fluorescein Dye | Fluorescein Dye 100 ppb | 10-506 |
| Purchase Fluorescein Dye 100 ppb PN 10-506. If required, dilute down to desired concentration with Deionized water. |
Optical Brighteners | Example: Purchased from Sigma Aldrich | Not Applicable |
| Can use any material under the optical brightener type. TIDE detergent is one example. |
Phycocyanin (Freshwater Cyanobacteria) | Rhodamine WT 200 ppb | 6500-020 | 210 ppb | Use our guide on our website: Turner Designs AquaFluor Handheld Fluorometer |
Phycoerythrin (Marine Cyanobacteria) | Rhodamine WT 100 ppb | 6500-020 | 950 ppb | Purchase 6500-020 and dilute to 50% with Deionized water |
Rhodamine Dye | Rhodamine WT Dye 200 ppb or 400 ppb | 6500-020 or 6500-120 |
| Purchase Rhodamine Dye. If required, dilute down to desired concentration with Deionized water. |
Turbidity | 10 NTU or 100 NTU | Sold by AMCO Clear Standards purchased from GFS Chemicals |
| Sold by AMCO Clear Standards purchased from GFS Chemicals |
Trilogy
Use our user manual: Trilogy User Manual (turnerdesigns.com)
Trilogy Application Optics | Associated Trilogy Module Part Number | Liquid Calibration Standard Concentration | Turner Designs Part Number for Calibration Solutions | Equivalent Fluorophore Concentration | Notes |
Ammonium | 7200-067-W |
| Not Applicable |
| Use our guide on website: Fluorometer Parameters | Ammonium | Turner Designs |
CDOM | 7200-069-W |
| Not Applicable |
| Calibrate using Quinine Sulfate or known concentrations of CDOM. Refer to website for example procedures: Fluorometer Parameters | CDOM | Turner Designs |
Chlorophyll a Extracted - Acidification | 7200-040-W | Chlorophyll a standards | 10-850 | Based on Certificate | Using the 10-850, calibrate the module based on values on the certificate. Another option, chlorophyll standards made from reconstituted powder using the EPA 445 method is a viable option. |
Chlorophyll a Extracted - Non Acidification | 7200-046-W | Chlorophyll a standards | 10-850 | Based on Certificate | Using the 10-850, calibrate the module based on values on the certificate. Another option, chlorophyll standards made from reconstituted powder using the EPA 445 method is a viable option. |
Chl in vivo | 7200-043 |
| Not Applicable |
| Use of known concentration primary standards is preferred |
Fluorescein Dye | 7200-048 | Fluorescein Dye 100 ppb | 10-506 |
| Purchase Fluorescein Dye 100 ppb PN 10-506. If required, dilute down to desired concentration with Deionized water. |
Histamine | 7200-049 |
| Not Applicable |
| Calibration determined by customer dictated application. Refer to guidelines set by your company. |
Nitrate | 7200-074 |
| Not Applicable |
| Refer to documentation our website for examples: Fluorometer Parameters | Absorbance | Turner Designs |
Optical Brighteners | 7200-047-W | Example: Purchased from Sigma Aldrich | Not Applicable |
| Can use any material under the optical brightener type. TIDE detergent is one example. |
Phosphate | 7200-070 |
| Not Applicable |
| Refer to documentation our website for examples: Fluorometer Parameters | Absorbance | Turner Designs |
Phycocyanin | 7200-044-W |
| Not Applicable |
| Use of known concentration primary standards is preferred |
Phycoerythrin | 7200-042-W |
| Not Applicable |
| Use of known concentration primary standards is preferred |
Rhodamine Dye | 7200-042-W | Rhodamine WT Dye 200 ppb or 400 ppb | 6500-020 or 6500-120 |
| Purchase Rhodamine Dye. If required, dilute down to desired concentration with Deionized water. |
Silicate | 7200-072 |
| Not Applicable |
| Refer to documentation our website for examples: Fluorometer Parameters | Absorbance | Turner Designs |
Turbidity | 7200-060 | 10 NTU, 100 NTU, or 1000 NTU | Sold by AMCO Clear Standards purchased from GFS Chemicals |
| Sold by AMCO Clear Standards purchased from GFS Chemicals |