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Calibration Solutions for Various Instruments
Calibration Solutions for Various Instruments

Calibration Solution Fluorophore Correlation and Articles

Updated over a month ago

FluoroSense

FluoroSense User Manual: 998-2852.pdf (turnerdesigns.com)

Name of Instrument

Instrument Part Number

Turner Designs Part Number for Calibration Solutions

Part Description

FluoroSense CHLOROPHYLL

2860-000-C

6500-220

FLUOROSENSE - CHLOROPHYLL uses RHODAMINE WT DYE, 800 PPB, 1 LITER

FluoroSense PHYCOCYANIN

2860-000-P

6500-020

FLUOROSENSE - PC, PHYCOCYANIN uses RHODAMINE WT DYE, 200 PPB, 1 LITER

Cyclops-7F, C3, or C6P

Cyclops-7F, C3, and C6p Calibration Guide: Calibration guide.pdf

Cyclops-7F User Manual: 998-2100 Rev 4.0 (turnerdesigns.com)
C3 and C6p User Manuals: 998-2300.pdf (turnerdesigns.com)

Cyclops-7F, C3, or C6P Application Optics

Liquid Calibration Standard Concentration

Turner Designs Part Number for Calibration Solutions

Equivalent Fluorophore Concentration

Notes

Chl in vivo (Blue)

Rhodamine WT 400 ppb

6500-120

40 ug/L

Use our guide on our website: Turner Designs Cyclops-7 Submersible Sensors

Phycocyanin (PC)

Rhodamine WT 200 ppb

6500-020

260 ppb

Use our guide on our website: Turner Designs Cyclops-7 Submersible Sensors

Phycoerythrin (PE)

Rhodamine WT 50 ppb

6500-020

500 ppb

Diluted from 6500-020 to 50 ppb (1 part dye to 3 parts deionized water) before use with Calibration Instructions 1.3 for PE units.

Use our guide on our website: Turner Designs Cyclops-7 Submersible Sensors

Crude Oil

PTSA 100 ppb

10-608

275 ppm

Osberg Light Crude Oil

Use our guide on our website: Turner Designs Cyclops-7 Submersible Sensors

CDOM

PTSA 400 ppb

10-609

125 ppb

Use our guide on our website: Turner Designs Cyclops-7 Submersible Sensors

Turbidity

10 NTU, 100 NTU, or 1000 NTU

Sold by AMCO Clear Standards purchased from GFS Chemicals

Sold by AMCO Clear Standards purchased from GFS Chemicals

Use our guide on our website: Turner Designs Cyclops-7 Submersible Sensors

Tryptophan

5000 ppb

Sold by Sigma Aldrich, CAS NO. 73-22-3

Weigh out 0.1g of tryptophan into 1L ultrapure water to make 100mg/L solution (100,000ppb). Then, 50mL of the 100mg/L is transferred to another 1L volumetric flask and made up with ultrapure water to get 5000ug/L (5000ppb).

Use our guide on our website: Turner Designs Cyclops-7 Submersible Sensors

Refined Fuels

4500 ppb NDSA

Sold by Fisher Scientific, CAS NO. 76758-30-0

1 ppm BTEX

Use our guide on our website: Turner Designs Cyclops-7 Submersible Sensors

Optical Brighteners

Can use any material under the optical brightener type. TIDE detergent is one example.

Use our guide on our website: Turner Designs Cyclops-7 Submersible Sensors

AquaFluor

AquaFluor Application Optics

Liquid Calibration Standard Concentration

Turner Designs Part Number for Calibration Solutions

Equivalent Fluorophore Concentration

Notes

Ammonium

Not Applicable

CDOM

Not Applicable

Calibrate using Quinine Sulfate or known concentrations of CDOM. Refer to website for example procedures: Fluorometer Parameters | CDOM | Turner Designs

Chl in vivo

Rhodamine WT 400 ppb

6500-120

60 ug/L

Use our guide on our website: Turner Designs AquaFluor Handheld Fluorometer

Crude Oil

PTSA 100 ppb

10-608

145 ppm

Oil Emulsions in water
Use our guide on our website: Turner Designs AquaFluor Handheld Fluorometer

Fluorescein Dye

Fluorescein Dye 100 ppb

10-506

Purchase Fluorescein Dye 100 ppb PN 10-506. If required, dilute down to desired concentration with Deionized water.

Optical Brighteners

Not Applicable

Can use any material under the optical brightener type. TIDE detergent is one example.

Phycocyanin (Freshwater Cyanobacteria)

Rhodamine WT 200 ppb

6500-020

210 ppb

Use our guide on our website: Turner Designs AquaFluor Handheld Fluorometer

Phycoerythrin (Marine Cyanobacteria)

Rhodamine WT 100 ppb

6500-020

950 ppb

Purchase 6500-020 and dilute to 50% with Deionized water

Use our guide on our website: Turner Designs AquaFluor Handheld Fluorometer

Rhodamine Dye

Rhodamine WT Dye 200 ppb or 400 ppb

6500-020 or 6500-120

Purchase Rhodamine Dye. If required, dilute down to desired concentration with Deionized water.

Turbidity

10 NTU or 100 NTU

Sold by AMCO Clear Standards purchased from GFS Chemicals

Sold by AMCO Clear Standards purchased from GFS Chemicals

Trilogy

Trilogy Application Optics

Associated Trilogy Module Part Number

Liquid Calibration Standard Concentration

Turner Designs Part Number for Calibration Solutions

Equivalent Fluorophore Concentration

Notes

Ammonium

7200-067-W

Not Applicable

CDOM

7200-069-W

Not Applicable

Calibrate using Quinine Sulfate or known concentrations of CDOM. Refer to website for example procedures: Fluorometer Parameters | CDOM | Turner Designs

Chlorophyll a Extracted - Acidification

7200-040-W

Chlorophyll a standards

10-850

Based on Certificate

Using the 10-850, calibrate the module based on values on the certificate. Another option, chlorophyll standards made from reconstituted powder using the EPA 445 method is a viable option.

Chlorophyll a Extracted - Non Acidification

7200-046-W

Chlorophyll a standards

10-850

Based on Certificate

Using the 10-850, calibrate the module based on values on the certificate. Another option, chlorophyll standards made from reconstituted powder using the EPA 445 method is a viable option.

Chl in vivo

7200-043

Rhodamine WT 400 ppb

6500-120

45 ug/L

Use our user manual page 20: Trilogy User Manual (turnerdesigns.com)

Fluorescein Dye

7200-048

Fluorescein Dye 100 ppb

10-506

Purchase Fluorescein Dye 100 ppb PN 10-506. If required, dilute down to desired concentration with Deionized water.

Histamine

7200-049

Not Applicable

Calibration determined by customer dictated application. Refer to guidelines set by your company.

Nitrate

7200-074

Not Applicable

Refer to documentation our website for examples: Fluorometer Parameters | Absorbance | Turner Designs

Optical Brighteners

7200-047-W

Not Applicable

Can use any material under the optical brightener type. TIDE detergent is one example.

Phosphate

7200-070

Not Applicable

Refer to documentation our website for examples: Fluorometer Parameters | Absorbance | Turner Designs

Phycocyanin

7200-044-W

Rhodamine WT 200 ppb

6500-020

2000 ppb

Use our user manual page 20: Trilogy User Manual (turnerdesigns.com)

Phycoerythrin

7200-042-W

Rhodamine WT 100 ppb

6500-020

1012 ppb

Purchase 6500-020 and dilute to 50% with Deionized water.

Use our user manual page 20: Trilogy User Manual (turnerdesigns.com)

Rhodamine Dye

7200-042-W

Rhodamine WT Dye 200 ppb or 400 ppb

6500-020 or 6500-120

Purchase Rhodamine Dye. If required, dilute down to desired concentration with Deionized water.

Silicate

7200-072

Not Applicable

Refer to documentation our website for examples: Fluorometer Parameters | Absorbance | Turner Designs

Turbidity

7200-060

10 NTU, 100 NTU, or 1000 NTU

Sold by AMCO Clear Standards purchased from GFS Chemicals

Sold by AMCO Clear Standards purchased from GFS Chemicals

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