Yes - different customers can have different prices.
In Wholesale Now, you would need to create one discount group per customer and then add the customer-specific prices to that discount group. Customers would then need to be tagged with the tag for their respective discount group.
Please note though that Wholesale Now stores settings in your theme, and theme files are limited to 256kb in size. This limits the number of discount groups Wholesale Now can work with.
If you need to create different prices for more than say 20 customers then Wholesale Now might not be the best fit due to storage limitations.