Get started
Read the introduction to this series of tutorials, then complete all of the previous tutorials in the series. For more information, click here.
Create a copy of the workflow that you created in Core elements tutorial 1.2. To learn how, click here.
Rename the copy Core elements tutorial 1.3. To learn how, click here.
Allocate the liquids to some plates
In the workflow called Core elements tutorial 1.3, turn on the Define Plates input to expose some inputs that you can use to define your plates.
Under Plate Names, define the name that you would like to give to your plate. Selecting Add New Item will allow you to define multiple plates.
Click Edit Plate Contents to open the Plate Contents Editor in which you can specify the plate type and which liquids should be in which wells. In Plate Types, select a plate type.
Using the plate map in the Plate Contents Editor, click and drag the cursor over the wells to which you want to allocate your samples, then click the range that Synthace displays on the right.
Specify that you want Synthace to allocate the samples to the selected wells, the volume that you want the wells to contain, the pattern and the order in which you want Synthace to allocate the samples to the wells, and the number of replicates that you want, then click Done.
Tip: You can specify the name of a liquid to allocate that liquid to the selected wells, the name of a tag to allocate every liquid with that tag to the selected wells, or default to assign every other liquid to the selected wells.
6. Click and drag the cursor over the wells to which you want to allocate your
standards, click the range that Synthace displays on the right, specify how you
want Synthace to allocate the standards to the wells, then click Done.
7. We want Synthace to treat the wells that contain the liquids with the same name the
same way. This allows Synthace to transfer that liquid from any of the wells that
contain it. To do this, turn off Treat Duplicate Names As Distinct Liquids.
Tip: If you want Synthace to auto-allocate any additional liquids onto this plate, turn on the Allow Auto-Allocation of Extra Liquids On Plate input. In this example, we do not want Synthace to do this.
Simulate the workflow
Check that the device that you selected can follow the instructions that you prepared. To learn how, click here.
Preview the execution
After you simulate the workflow, click View Simulation to open the simulation details.
Open the Preview tab, then click through the steps to check that the instructions that Synthace has generated are correct.
Check your work:
To see what your finished workflow to this tutorial should look like simply navigate to the Tutorials and search for Tutorial: 1. Define Liquids and Plates.
Challenges
Try to compare how the aliquoting behaviour changes when you select different available options for Well Iteration Pattern in the Plate Contents Editor. How do Typewriter, Snake and 4 well grid and 96 well quadrant alter the layout and liquid handling.