Get started
Read the introduction to this series of tutorials, then complete all of the previous tutorials in the series. For more information, click here.
Create a workflow in the builder. To learn how, click here.
Rename the workflow Core elements tutorial 1.4. To learn how, click here.
Select the device on which you want to run your workflow. To learn how, click here.
Define the liquids
Open the Elements panel, search for Define Liquids And Plates, then click the element to add it to the canvas.
On the canvas, click the element to open a list of inputs to the element (i.e., a list of things that you can control).
From the Define From input, select Previous Simulation to expose some inputs that you can use to define your liquids.
In the Plates input, click Add New Item, then Plate From A Simulation.
Use the Simulations form to select the simulation that contains the plate that you want.
Tip: In this example, we have selected the simulation that we created in Core elements tutorial 1.2. You might want to do the same.
When you select a simulation, Synthace displays every plate in the simulation. Select the plate that you want to view its contents (e.g., Plate A: the output plate from the previous tutorial), then click Select to return to the builder.
This populates the Plates input with the name of the plate that you selected.
When you retrieve a liquid from a previous simulation, you can specify a new liquid policy.
Add a new entry, specify the liquid (or liquids) for which you want to specify a new liquid policy, click Select A Policy to open the liquid policies library, then select a new liquid policy.
Specify the following Plate Options.
Aliquot the liquids to a new plate
We have retrieved 28 liquids from a previous simulation (8 x Liquid A, 8 x Liquid B, 8 x Liquid C and 4 x Liquid D) all of which we are going to treat as distinct liquids.
Let's instruct a device to transfer a single aliquot of each one to a new plate.
Open the Elements panel, search for Aliquot, then click the element to add it to the canvas.
We now need to pass the liquids that we defined in the Define Liquids And Plates element to the Aliquot element, so that the Aliquot element knows which liquids to aliquot.
To do so, click and drag the Liquids output from the Define Liquids And Plates element to the Liquids To Aliquot input of the Aliquot element.
Click the Aliquot element, then specify the Aliquot Volume and Replicates.
Specify a Plate Set Name and a Plate Type.
Simulate the workflow
Check that the device that you selected can follow the instructions that you prepared. To learn how, click here.
Preview the execution
After you simulate the workflow, click View Simulation to open the simulation details.
Open the Preview tab, then click through the steps to check that the instructions that Synthace has generated are correct.
Note: Because we specified that we wanted to treat liquids with the same name as distinct liquids, Synthace aliquots each liquid once, it does not create a single aliquot per named liquid.