In this tutorial you will learn how to ensure liquid volumes are consistent across samples by normalizing the total volumes of a set of liquids in a plate.
Get started
Read the introduction to this series of tutorials, then complete all of the previous tutorials in the series. For more information, click here.
Create a workflow in the builder. To learn how, click here.
Rename the workflow Core elements tutorial 4.3. To learn how, click here.
Select the device on which you want to run your workflow. To learn how, click here.
Select a default plate type. To learn how, click here.
Define your samples and workflow structure
In the Workflow Builder add three elements to your canvas: 2 x Define Liquids And Plates and 1 x Normalize. Rename these elements to
Define BiomassandDefine Diluent.Connect the output Liquids parameter from Define Biomass into Liquids To Normalize of Normalize element.
Connect the output Liquids parameter from Define Diluent into the Diluents input of Normalize.
Define your Samples that are intended for normalization. For more information on how, see here. As this normalization is in-place the parameters Plate Name, Plate Type, Location and Volume are required.
Define your Diluent.
Define liquid handling behaviour
In the Normalize element select the In Place option in the Normalization Location parameter and By Concentration option in Normalization Mode.
Specify which diluent to use for each normalization in Diluent To Use.
In the Normalization Options specify the Target Volume
Simulate the workflow
Check that the device that you selected can follow the instructions that you prepared. To learn how, click here.
Preview the execution
After you simulate the workflow, click View Simulation to open the simulation details.
Open the Preview tab, then click through the steps to check that the instructions that Synthace has generated are correct.
You will see that diluent is added to each well containing your samples to normalize the final volume of all the samples.
Check your work:
To see what your finished workflow to this tutorial should look like simply navigate to the Tutorials and search for Tutorial: 4. Dilute.
