Contents
Match your labware
In preparation for your device deployment, you will be asked to provide some information on the labware you wish you use on your device.
Plate name in Vendor Software
Part Number
Vendor Name/ Manufacturer
Synthace Plate Library ID
Please specify what you'd like us to name them; this is how they will appear in the Synthace Plate LibraryMaximum well volume (μl)
Residual well volume (μl)
In order to properly generate device instructions using your labware in Fluent Control it is important that two fields in Fluent Control are matched with fields in Synthace.
Example parameters for the labware you might use in your Synthace environment are listed in the table below. It is essential that these parameters match exactly, including white spaces and capitalisation.
Synthace Plate Name | Vendor Name /Manufacturer | Part Number |
50 mL Falcon Tube | Eppendorf | 30122151 |
15 mL Falcon Tube | Eppendorf | 30122178 |
2.0 mL Corning Tube | Corning | 430659 |
Plate Reservoir | Agilent | 201250-100 |
PCR Plate | BioRad | MSP9601 |
96 deep well plate | Corning | P-DW-20-C-S |
12 deep well plate | Agilent | 204095-100 |
24 deep well plate | VWR | 89080-534 |
96 well plate | Thermo-Fisher | 44-2404-21 |
Robocolumn Long | Repligen | RoboLong |
Robocolumn Short | Repligen | RoboShort |
Tecan Trough 100 ml | Tecan | 10613049 |
Please add these Vendor Name and Part Numbers that you provided to Synthace, in the fields in the labware definition within Fluent Control.
These can be found by opening the labware in Fluent Control under the “Miscellaneous” section.
Import the Synthace liquid classes
Please import the Synthace liquid classes provided to you by your Field Application Scientist, via the Fluent Control database import tool. These have been thoroughly tested in the lab, and are used by Synthace to ensure that all types of transfer (free, contact and wet dispensing) can be simulated and performed correctly.
! Ensure that the “Import device placements” is OFF!
Review your labware and RGA vectors
Ahead of the deployment please review labware and RGA vector teaching to ensure that we can test the instructions generated by Synthace. This includes teaching for dispensing into robocolumns. The key RGA vectors to check are:
TeShuttle transport
Nests
Hotels
TeChrom waste tray engage and disengage
Plate Reader
Review your carrier definitions
Please review the carrier definitions and ensure the allowed labware on the carriers matches up with what you require. Ensure that the RGA grip vectors match up with site accessibility on your deck. This may require you to duplicate some of the nests on the deck and modify the allowed grip types and allowed labware.
If you intend to use a mixture or micro-well and deep-well collection plates, it is particularly important that the allowed deck positions for your labware are correctly configured to avoid any collisions when trying plates placed next to deepwell plates. The best way to do this is to control the locations that can have deep-well plates placed on them by the user. The image below shows an overview of how we have set up grip positions and allowed labware in the Synthace lab.
If you are planning to use plate hotels in your workflow please also ensure you have an RGA re-grip position setup on your deck.