This article teaches you how to execute a robocolumns workflow on a Tecan FLUENT®.

For more information on the robocolumns elements, click here.

For more information on building robocolumns workflows, click here.

Once you have completed your workflow, click Simulate. When you view your simulation, the Simulation details tab will provide you with an overview of the labware, and reagents necessary to execute the workflow.

The Setup tab provides information on the plate name, which reagent is present in the plate and its volume.

The Preview tab shows where Synthace has allocated labware on your FLUENT deck. Ensure that the proper labware is placed in the exact position as shown in the preview.

Tip: To quickly check plate contents, hover over the well with your cursor.

Ensure that the simulation is behaving as expected. Use the arrows on your keyboard or drag the preview bar with your mouse to check each step in your workflow.

Once you have confirmed the simulation is functioning properly, click Schedule in the upper right hand corner. This will send the device instruction of the workflow to SynthaceHub.

Launch SynthaceHub on the PC connected to your Fluent. When you open the home page, there will be an available execution with the same name as the simulated workflow.

Toggle to the Executions tab.

If you wish to double check placement of input labware or starting reagent volumes, click on the Show preview button.

Launch Fluent Control and ensure that no worktables are loaded. Synthace controls the Fluent via an API method in Fluent Control.

Once you are ready to execute, hit the start button. The Device Plugin will launch. Then click Start from selected line and the execution will start.

Tip: If it is your first time starting the execution, start from Line No 1. If you wish to start from a different command for troubleshooting or error recovery, select the proper line number and start the execution.