Introduction
Synthace integrates with Tecan’s Spark Series plate reader by reading the data files generated by the Spark Control software and associating them to a plate in a Synthace execution. Data files must be exported from Spark Control and linked to the associated plates directly.
Data export configuration
In order for Synthace to correctly read the data files generated by Spark Control, some Data handling settings must be configured in Spark Control:
Within Spark Control, set the Destination to: New workbook
Configure the Output → Endpoint as follows:
Export mode: List
List orientation: Horizontal
Configure the Output → Kinetic as follows:
Export mode: List
List orientation: Horizontal
Time: Time per cycle
Supported method strips
Detection modes
The following modes are supported:
Absorbance
Multiple reads per well must be set to Not defined
If pathlength correction is enabled, the Correction factor must be set to Manual
Fluorescence intensity
Multiple reads per well must be set to Not defined
Gain must NOT be set to Extended dynamic range
Luminescence
All configurations supported.
Note that the Scan counterparts to the below detection modes are not currently supported.
Kinetic loops
The kinetic loop strip allows for detection mode readings to be repeated. Kinetic loop strips are supported by Synthace.
Actions
Any action strips included in the Spark Control method will be ignored by Synthace.
Next steps
Once you have selected the correct data export settings and you have generated some data files, you will want to add data to your runs. See the “How to Add Data From a Supported Device Using a Local File” section from Adding data to your runs for more information.