When your business receives a dispute (also called a chargeback), the payment amount, as well as a separate dispute fee levied by the card network, will be deducted from your account balance.
Card networks apply this additional dispute fee to help protect cardholders and discourage dishonest or fraudulent business practices that could lead to chargebacks. The network fees for chargebacks are imposed regardless of whether you choose to dispute the chargeback, and regardless of the outcome of any given dispute. However, in the event that a dispute is ruled in your favor, Stripe will refund this fee to you at our expense.
The fee for disputes varies by region, and you can see more on the Stripe pricing page.