Core elements tutorial 7.1
In some experimental protocols, you might have two sets of liquids, standards and samples, for example, which are initially handled separately, but need to be treated the same way afterwards. For example, you might have two groups of liquids in different plates, or you might have a set of liquids which is diluted and another which isn’t, but at one point you might want to aliquot them in the same way to the same plate. To do this, you want to group the liquids together, or append them, which can be done using the Append element.
Get started
Append two sets of liquids and aliquot all
In this example, one set of liquids is defined by the Define Liquids and Plates element, and the other by the Aliquot element. The two sets are appended, and aliquoted together.
In the workflow builder, add 5 elements to your canvas: 2x Define Liquids and Plates, 2x Aliquot and 1x Append. Define the two sets of liquids and the first set of aliquots. To learn how, click here.
Connect the Aliquots parameter from the Aliquot element to the Liquids A input parameter of the Append element.
Connect the Liquids output parameter from the Define Liquids and Plates element to the Liquids B input parameter of the Append element.
Connect the Liquids output parameter from the Append element to the Liquids to Aliquot input parameter of the Aliquot element.
Make the final connection between Define Liquids and Plates and Aliquot
Specify the Aliquot Volume, Replicates, Plate Name and the Plate Type parameters in the Aliquot element.
Note: To apply a parameter value to all input liquids use the default identifier, as in the example above. To apply a parameter value to a specific liquid then provide the liquid name, or alternatively use a tag to apply a parameter value to a group of liquids. This behaviour is true for most parameters unless stated otherwise.
Simulate the workflow
1. Check that the device that you selected can follow the instructions that you prepared. To learn how, click here.
Preview the execution
1. After you simulate the workflow, click View Simulation to open the simulation details.
2. Open the Preview tab, then click through the steps to check that the instructions that Synthace has generated are correct.
Check your work:
To see what your finished workflow to this tutorial should look like simply navigate to the Tutorials and search for Tutorial: 7. Utility elements.
