Get started
Read the introduction to this series of tutorials. For more information, click here.
Create a workflow in the builder. To learn how, click here.
Rename the workflow Core elements tutorial 6.1. To learn how, click here.
Select the device on which you want to run your workflow. To learn how, click here.
Select a default plate type. To learn how, click here.
Note: Multiple input plates can be specified, and the input liquids will be allocated to the plate that best fits its volume requirements. In this case, we expect to need a large amount of Medium, which can be added to a reservoir, while the additives can be added to a trough.
Add medium and additives to different cell lines
In this example, we will take cultures from six different cell lines and aliquot them in four replicates to a 24-well plate. We will then add medium and two additives to all wells containing the cell culture.
Add two Define Liquids and Plates elements to the builder. In one, define your cell lines, and in the other, define the medium and the additives. Rename the instances Define Cell lines and Define Medium and additives, respectively. For more information on how to use the Define Liquids and Plates, click here.
Now aliquot your cells to a 24-well plate using the Aliquot element. For this, add an Aliquot element to the builder, and connect the Liquids output in Define Liquids and Plates to Liquids to Aliquot in the Aliquot element.
Click on the Aliquot element, to open the parameter editor. Specify the aliquot volume, number of replicates and replicate grouping.
Specify your plate type and layout. For more information click here.
Now add a Mix Onto element to the builder.
We want to add the medium and additives onto the Aliquot plate, so connect the Aliquots output of the Aliquot element to the Destination Liquids input of the Mix Onto element. Then connect the Liquids output of the Define Liquids and Plates element where the medium and additives are defined to the Source Liquids input of the Mix Onto element.
Click on the Mix Onto element to open the element parameter editor. In this case, we want all cell lines to get the same medium and additives, so we specify the addition mode to Mix All Onto All.
The new mixtures can inherit the names of the liquids already on the plates, in this case the cell lines, or you can specify a new name. In this case, we will not rename the mixtures so select the option Do Not Rename.
Now, we can define the volume of each reagent that should be added to the cell lines.
Simulate the workflow
Check that the device that you selected can follow the instructions that you prepared. To learn how, click here.
Preview the execution
After you simulate the workflow, click View Simulation to open the simulation details.
Open the Preview tab, then click through the steps to check that the instructions that Synthace has generated are correct.
You can see that the cell lines are aliquoted in replicates of four, and then Medium, Additive A and Additive B are added onto all the aliquots. In this case, the order of the addition of the reagents is specified by their order in the Define Liquids and Plates element.
Check your work:
To see what your finished workflow to this tutorial should look like simply navigate to the Tutorials and search for Tutorial 6: Mix Onto.
Challenges
In the example above, the resulting liquids retain the original name of the cell lines. Explore the different naming options, including applying different prefixes to different cell lines based on their name or tag.