Get started
Read the introduction to this series of tutorials, then complete all of the previous tutorials in the series. For more information, click here.
Create a workflow in the builder. To learn how, click here.
Rename the workflow Core elements tutorial 6.2. To learn how, click here.
Select the device on which you want to run your workflow. To learn how, click here.
Select a default plate type. To learn how, click here.
Note: Multiple input plates can be specified, and the input liquids will be allocated to the plate that best fits its volume requirements. In this case, we expect to need a large amount of Medium, which can be added to a reservoir, while the additives can be added to a trough.
Add different additives to each cell line
In this example, we will take cultures already in a 24-well plate (6 different cell lines, four replicates of each), and to each one add a different additive, in replicates (6 additives), in the order in which they are provided. We are matching each cell line to each additive.
Add two Define Liquids and Plates elements to the builder. In one, define your cell lines, and in the other, define the additives. Rename the instances Define Cell lines and Define additives, respectively. For more information on how to use the Define Liquids and Plates click here.
Now add a Mix Onto element to the builder. We want to add the additives onto the plate with the cell cultures, so connect the Liquids output of the Define Liquids and Plates element where the cell lines are defined to the Destination Liquids input of the Mix Onto element. Then, connect the Liquids output of the Define Liquids and Plates element where the additives are defined to the Source Liquids input of the Mix Onto element.
Click on the Mix Onto element to open the element parameter editor. In this case, we want each cell line to receive one unique additive, so we specify the addition mode to Mix In Order.
The new mixtures can inherit the names of the liquids already on the plates, in this case the cell lines, or you can specify a new name. In this case, we will not rename the mixtures so select the option Do not rename.
Now, we can define the volume of each reagent that should be added to the cell lines. In this case, all additives should be added with the same volume, so specify a default value.
Importantly, you can specify a number of replicates you want each liquid to be added. In this case, since the cell lines are in groups of four replicates and we want each replicate to get the same additive, set the replicate number to 4 for all additives.
Simulate the workflow
Check that the device that you selected can follow the instructions that you prepared. To learn how, click here.
Preview the execution
After you simulate the workflow, click View Simulation to open the simulation details.
Open the Preview tab, then click through the steps to check that the instructions that Synthace has generated are correct.
You can see that the cell lines are already distributed in the 24-well plate in groups of four replicates along columns, and to each one a specific additive is added. In the end, cell line 1 received additive A, cell line 2 additive B, and so forth.