This guide provides a clear overview of the steps involved in automating a protocol using Synthace, from creating your experimental process to executing it.
Create
The first step is to design your Synthace workflow that can be executed in your lab. There are a number of type of workflow which can be used to create liquid handling instructions in Synthace, the Builder, Cherry Picker, or Form to prepare instructions for your lab devices. The most common method is using the Builder, and so this article will focus on this method of experimental design
Example: The image below shows a builder workflow, consisting of steps to automate a protocol for a simple protein quantification assay using a liquid handler.
Simulate
When you simulate a workflow, Synthace verifies that your lab devices can follow the prepared instructions. It translates these instructions into a series of executable steps, calculating necessary details such as:
Input liquids needed
Transfer volumes
Liquid class selection
Labware types needed
Well locations for input and output liquids
Output liquid concentrations
Simply hit the simulate button to perform this translation.
Preview
Open the simulation in the Simulation Details page and use the Preview tab to visualize the instructions. This helps ensure the instructions will produce the desired outcome.
If adjustments are needed:
Go back to the Builder.
Open and modify the workflow.
Simulate the workflow again.
Start Execution
Once satisfied with the instructions, click the Start Execution button. This creates an Execution entity in Synthace, consisting of executable tasks broken down into stages.
Prepare
Prepare the samples, buffer, and reagents required by your workflow. Use the Reagent Stock Calculator in the Execution page to define master stock solutions, and Synthace will provide preparation instructions.
Check the Simulation Details page for input plate preparation details. Use the Setup tab for volume additions and the Preview tab for deck positions.
The setup and preview page can be accessed directly from the execution stages
Schedule
Transfer your device instructions to your device. The process varies based on your execution mode:
For automated workflows using liquid handlers, click Schedule to send the instructions to the device.
Execute
When ready to run the execution, use SynthaceHub to send the instructions to the applications controlling your lab devices.
